Development and Application of an iELISA for the Detection of Antibody against Salmonella Abortusequi

Equine abortus salmonellosis is a bacterial disease that causes high abortion rates in susceptible equids and therefore significant economic losses. Although the tube agglutination test (TAT) commonly used serological for S. Abortusequi, it not highly specific or sensitive, development of more rapid assays urgently required. In this study, an indirect enzyme-linked immunosorbent assay (iELISA) was developed detection flagellum protein (FljB) antibodies against Abortusequi. Negative sera from horses China (n = 1030) were to establish baseline negative population, reference antisera positive other viruses bacteria cross reactivity technique. The performance FljB iELISA evaluated standard TAT, tested using field serum samples. 8–16 times sensitive than TAT. ROC analysis showed accurate, with area under curve (AUC) 0.9943 (95% CI, 0.9815–1.000). diagnostic sensitivity (DSe) 98.9% Cl, 93.84%–100.00%), which higher TAT (DSe 38.6; 95% 29.14%–49.08%). specificity (DSp) 100.0% 95.82%–100.0%). When 508 clinical samples tested, had rate 51.38% (261/508, 51.24%–51.51%), (44/508). We also performed survey Abortusequi infection, series collected across eighteen provinces 2021. results all except Jiangsu certain number cases, ranged 0% 96.9%, indicating wide spread China. abovementioned suggest study rapid, specific, repeatable likely be suitable large-scale surveys control infection.